Recombinant Human PP1 alpha/PPP1A His Protein

Name: Recombinant Human PP1 alpha/PPP1A His Protein
Brand: Novus Biologicals
SKU: NBP1-72418

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72418

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

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Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-330 of Human PP1 alpha/PPP1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PP1 alpha/PPP1A His Protein

SDS-PAGE: Recombinant Human PP1 alpha/PPP1A His Protein [NBP1-72418]

SDS-Page: Recombinant Human PP1 alpha/PPP1A Protein [NBP1-72418] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72418

Formulation	50mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50% glycerol
Preservative	No Preservative
Concentration	0.2 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP1 alpha

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Protein Phosphatase 1 Catalytic Subunit alpha

Alternate Names

PPP1A, PPP1CA

Gene Symbol

PPP1CA

Additional PP1 alpha Products

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COA

Product Specific Notices for Recombinant Human PP1 alpha/PPP1A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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