Key Product Details
The ED50 for this effect is 6-60 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002) and 1-3 ng/mL when measured by 3H-thymidine incorporation.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.|
Reconstitute at 50 µg/mL in sterile 4 mM HCl.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems’ hPDGF consists predominantly of hPDGF-AB heterodimers. PDGF obtained from porcine platelets contain only PDGF-BB homodimers.
Two distinct PDGF receptors, the alpha-receptor and the beta-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha-receptor binds both the A and B chains with high affinity whereas the beta-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.
In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.
Entrez Gene IDs
Product Specific Notices for Porcine PDGF Protein, CF
For research use only