Recombinant Human POMC His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72334

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NBP1-72334-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all POMC Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-267 of Human POMC

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MWCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSS GSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE FKRELTGQRL REGDGPDGPA DDGAGAQADL EHSLLVAAEK KDEGPYRMEH FRWGSPPKDK RYGGFMTSEK SQTPLVTLFK NAIIKNAYKK GE

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human POMC His Protein

SDS-PAGE: Recombinant Human POMC His Protein [NBP1-72334]

SDS-PAGE: Recombinant Human POMC His Protein [NBP1-72334]

SDS-Page: Recombinant Human POMC Protein [NBP1-72334] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-72334
Formulation 20 mM Tris-HCl buffer (pH 8.5), 1 mM DTT, 50% glycerol, 0.2 M NaCl, 0.1 mM PMSF, 100 mM Imidazole
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POMC

Pro-opiomelanocortin preproprotein, also known as POMC, a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. It regulates the corticosteroid production in the adrenal cortex. Also, POMC is cleaved into ten hormone chains named NPP, gamma-MSH, ACTH, alpha-MSH, CLIP, Lipotropin beta, Lipotropin gamma, beta-MSH, beta endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency. Recombinant human POMC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proopiomelanocortin

Alternate Names

ACTH, adrenocorticotropic hormone, adrenocorticotropin, alpha-melanocyte-stimulating hormone, alpha-MSH, beta-endorphin, beta-LPH, beta-melanocyte-stimulating hormone, beta-MSH, CLIP, corticotropin-like intermediary peptide, Corticotropin-lipotropin, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, LPH, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, MSH, NPP, POC, pro-ACTH-endorphin, proopiomelanocortin, pro-opiomelanocortin, proopiomelanocortin preproprotein

Gene Symbol

POMC

Additional POMC Products

Product Documents for Recombinant Human POMC His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human POMC His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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