Recombinant Human PARD6B His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23315

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NBP2-23315
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PARD6B Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-372 of Human PARD6B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

43.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PARD6B His Protein

SDS-PAGE: Recombinant Human PARD6B His Protein [NBP2-23315]

SDS-PAGE: Recombinant Human PARD6B His Protein [NBP2-23315]

SDS-Page: PARD6B Protein [NBP2-23315]

Formulation, Preparation and Storage

NBP2-23315
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.2 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARD6B

Partitioning defective 6 homolog beta, also known as PARD6B, is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. It is expressed in pancreas and in both adult and fetal kidney, and is weakly expressed in placenta and lung. Recombinant human PARD6B protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

par-6 (partitioning defective 6, C.elegans) homolog beta, PAR-6 beta, par-6 partitioning defective 6 homolog beta (C. elegans), PAR6B, PAR-6B, partitioning defective 6 homolog beta

Gene Symbol

PARD6B

Additional PARD6B Products

Product Documents for Recombinant Human PARD6B His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PARD6B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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