Recombinant Rat Oncostatin M/OSM Protein

Name: Recombinant Rat Oncostatin M/OSM Protein
Brand: Novus Biologicals
SKU: NBP2-35280

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35280

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Oncostatin M/OSM Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 214 amino acids corresponding to Oncostatin M/OSM Source: E. coli

Uniprot ID: Q65Z15

Amino Acid Sequence: KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Oncostatin M/OSM as determined by LAL method.

Predicted Molecular Mass

24.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Oncostatin M Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Oncostatin M/OSM Protein

SDS-PAGE: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]

SDS-Page: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]

Formulation, Preparation and Storage

NBP2-35280

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, 0.1 % Tween-80.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Oncostatin M/OSM

Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 215 amino acids and has a molecular mass of 22.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and murine OSM.

Alternate Names

MGC20461, oncostatin M, oncostatin-M

Gene Symbol

OSM

Additional Oncostatin M/OSM Products

Product Documents for Recombinant Rat Oncostatin M/OSM Protein

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COA

Product Specific Notices for Recombinant Rat Oncostatin M/OSM Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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