Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75953

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NBP2-75953-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Nucleoside Diphosphate Kinase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-143 of E. coli Nucleoside Diphosphate Kinase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

SDS-PAGE: Recombinant E. coli Nucleoside Diphosphate Kinase His Protein [NBP2-75953]

SDS-PAGE: Recombinant E. coli Nucleoside Diphosphate Kinase His Protein [NBP2-75953]

SDS-Page: Recombinant E. coli Nucleoside Diphosphate Kinase His Protein [NBP2-75953] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-75953
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleoside Diphosphate Kinase

ndk, also known as Nucleoside diphosphate kinase, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP. Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 2.7.4.6, Multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase, NDK, Nucleoside 5'-diphosphate phosphotransferase, Nucleoside diphosphokinase

Additional Nucleoside Diphosphate Kinase Products

Product Documents for Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli Nucleoside Diphosphate Kinase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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