Recombinant Human NRG1 beta2 Protein

Name: Recombinant Human NRG1 beta2 Protein
Brand: Novus Biologicals
SKU: NBP2-35099

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35099

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q02297

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all NRG1 beta2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 61 amino acids corresponding to NRG1 beta2 Source: E. coli

Uniprot ID: Q02297

Amino Acid Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of NRG1 beta2 as determined by LAL method.

Predicted Molecular Mass

7.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

NRG1 beta2 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/ml, corresponding to a specific activity of >2.0 x 10^5

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NRG1 beta2 Protein

SDS-PAGE: Recombinant Human NRG1 beta2 Protein [NBP2-35099]

SDS-Page: Human NRG1 beta2 Protein [NBP2-35099]

Formulation, Preparation and Storage

NBP2-35099

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: NRG1 beta2

Neuregulin 1 (NRG1) is in humans encoded by the NRG1 gene. It is one of four proteins belonging to the neuregulin family that act on the EGFR family of receptor. Neuregulin 1 is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. All NRG1 isoforms contain an EGF like domain (alpha or beta splice variant that differ in their C terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 is necessary for cardiac development, structural maintenance, and functional integrity of the heart. NRG1 and its receptor family ErbB can play a beneficial role in the treatment of chronic heart failure (CHF) by promoting survival of cardiac myocytes, improving sarcomeric structure, balancing Ca2+ homeostasis, and enhancing pumping function.

Alternate Names

ARIA, GGF2, HGL, HRG, HRG1, MST131, MSTP131, NDFheregulin, alpha (45kD, ERBB2 p185-activator), neuregulin 1, pro-NRG1, SMDF

Gene Symbol

NRG1

UniProt