Recombinant Human NEURL2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23277

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NBP2-23277
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NEURL2 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-285 of Human NEURL2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAASEP VDSGALWGLE RPEPPPTRFH RVHGANIRVD PSGTRATRVE SFAHGVCFSR EPLAPGQVFL VEIEEKELGW CGHLRLGLTA LDPASLAPVP EFSLPDLVNL GHTWVFAITR HHNRVPREGR PEAEAAAPSR PPTLLVEPYL RIEQFRIPRD RLVGRSRPGL YSHLLDQLYE LNVLPPTARR SRLGVLFCPR PDGTADMHII INGEDMGPSA RGLPAAQPLY AVVDVFASTK SVRLVQLEYG LPSLQTLCRL VIQRSMVHRL AIDGLHLPKE LKDFCKYE

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NEURL2 His Protein

SDS-PAGE: Recombinant Human NEURL2 His Protein [NBP2-23277]

SDS-PAGE: Recombinant Human NEURL2 His Protein [NBP2-23277]

SDS-Page: Recombinant Human NEURL2 His Protein [NBP2-23277] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP2-23277
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEURL2

NEURL2 plays an important role in the process of myofiber differentiation and maturation. This protein is probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, which mediates the ubiquitination of proteins. It probably contributes to catalysis through recognition and positioning of the substrate and the ubiquitin-conjugating enzyme. During myogenesis, NEURL2 controls the ubiquitination and degradation of the specific pool of CTNNB1/beta-catenin located at the sarcolemma. Recombinant human NEURL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

C20orf163, chromosome 20 open reading frame 163, dJ337O18.6, FLJ30259, MGC125934, MGC125935, neuralized homolog 2 (Drosophila), neuralized-like 2, neuralized-like 2 (Drosophila), neuralized-like protein 2, Ozz, Ozz-E3

Gene Symbol

NEURL2

Additional NEURL2 Products

Product Documents for Recombinant Human NEURL2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NEURL2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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