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Recombinant Bacteria NAD Nucleotidase Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18175

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NBP3-18175

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Description

Full-length NAD 5'-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa.

Source: E. coli

Uniprot ID: Q4QNY4

Purity

>98%, by SDS-PAGE

Predicted Molecular Mass

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

With AMP: 856 4 nmol of Ade/min/mg of protein
With NMN: 110,3 nmol of NR/min/mg of protein
With NAD: 5 4 nmol of Ade/min per mg of protein | 5 3 nmol of NR/min/mg of protein

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP3-18175
Formulation 5mM KH2PO4, pH 6.8, 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NAD Nucleotidase

NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi

Alternate Names

nadN

Gene Symbol

nadN

Additional NAD Nucleotidase Products

Product Documents for Recombinant Bacteria NAD Nucleotidase Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Bacteria NAD Nucleotidase Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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