Recombinant E. coli MYH His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22653

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NBP2-22653
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MYH Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-350 of E.coli MYH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli MYH His Protein

SDS-PAGE: Recombinant E. coli MYH His Protein [NBP2-22653]

SDS-PAGE: Recombinant E. coli MYH His Protein [NBP2-22653]

SDS-Page: Recombinant E. coli MYH Protein [NBP2-22653]

Formulation, Preparation and Storage

NBP2-22653
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYH

Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E.coil mutY protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 3.2.2, EC 3.2.2.-, EC 3.2.2.31, hMYH, MGC4416, MutY homolog, MUTYH, Muyth, MYH, MYHA/G-specific adenine DNA glycosylase

Gene Symbol

MUTYH

Additional MYH Products

Product Documents for Recombinant E. coli MYH His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli MYH His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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