Is this product useful for T cell stimulation assays?

Name: Recombinant Mycobacterium tuberculosis Ab85A His Protein
Brand: Novus Biologicals
SKU: NBP1-99035

The TB Ab85A Protein (NBP1-99035) has only been validated in SDS-PAGE. It is a recombinant protein (with a His-tag), and it has not been tested for any functional activity. Therefore, unfortunately, we do not know whether or not it would work in T cell stimulation assays, and this application is not covered by our guarantee. However, if you do test this recombinant protein in your functional assay, you could apply for our Innovator's Reward program, where in return for sharing your results (positive or negative) with us by submitting an online review, you would receive a discount voucher for 100% of the purchase price of the reviewed product. For more information, please contact us at innovators@novusbio.com for more information.

Recombinant Mycobacterium tuberculosis Ab85A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99035

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Mycobacterium tuberculosis Ab85A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 43-338 of Mycobacterium tuberculosis TB Ab85A

Source:Baculovirus

Amino Acid Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mycobacterium tuberculosis Ab85A His Protein

SDS-PAGE: Recombinant Mycobacterium tuberculosis Ab85A His Protein [NBP1-99035]

SDS-Page: TB Ab85A Protein [NBP1-99035]

Formulation, Preparation and Storage

NBP1-99035

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mycobacterium tuberculosis Ab85A

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Alternate Names

85 A, 85A, Ag85A, Antigen 85 A, Antigen 85 complex A, Antigen 85A, Fbp A, FbpA, Fibronectin Binding protein A, Mpt 44, Mpt44, Mycobacterium tuberculosis antigen 85A, Mycolyl transferase 85 A, Mycolyl transferase 85A, Rv3804c, Secreted antigen Ag85A

Additional Mycobacterium tuberculosis Ab85A Products

Product Documents for Recombinant Mycobacterium tuberculosis Ab85A His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Mycobacterium tuberculosis Ab85A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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