Recombinant Human MKK6/MEK6 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51949

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NBP2-51949-0.02mg
NBP2-51949-0.1mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MKK6 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 53-314 of Human MKK6/MEK6

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLEPIMELGR GAYGVVEKMR HVPSGQIMAV KRIRATVNSQ EQKRLLMDLD ISMRTVDCPF TVTFYGALFR EGDVWICMEL MDTSLDKFYK QVIDKGQTIP EDILGKIAVS IVKALEHLHS KLSVIHRDVK PSNVLINALG QVKMCDFGIS GYLVDEVAKE IDAGCKPYMA PERINPELNQ KGYSVKSDIW SLGITMIELA ILRFPYDSWG TPFQQLKQVV EEPSPQLPAD KFSAEFVDFT SQCLKKNSKE RPTYPELMQH PFF

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MKK6/MEK6 His Protein

SDS-PAGE: Recombinant Human MKK6/MEK6 His Protein [NBP2-51949]

SDS-PAGE: Recombinant Human MKK6/MEK6 His Protein [NBP2-51949]

SDS-Page: Recombinant Human MKK6/MEK6 His Protein [NBP2-51949] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-51949
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK6

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4).

Long Name

Mitogen-activated Protein Kinase Kinase 6

Alternate Names

MAP2K6, MEK6, PRKMK6, SAPKK3

Gene Symbol

MAP2K6

Additional MKK6 Products

Product Documents for Recombinant Human MKK6/MEK6 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MKK6/MEK6 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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