Recombinant Human MIS12 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23237

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NBP2-23237
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MIS12 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-205 of Human MIS12

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MIS12 His Protein

SDS-PAGE: Recombinant Human MIS12 His Protein [NBP2-23237]

SDS-PAGE: Recombinant Human MIS12 His Protein [NBP2-23237]

SDS-Page: MIS12 Protein [NBP2-23237]

Formulation, Preparation and Storage

NBP2-23237
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIS12

Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

2510025F08Rik, hMIS12, homolog of yeast Mis12, KNTC2AP, MGC2488, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog (S. pombe), MIS12, MIND kinetochore complex component, homolog (yeast), MTW1, protein MIS12 homolog

Gene Symbol

MIS12

Additional MIS12 Products

Product Documents for Recombinant Human MIS12 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MIS12 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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