Recombinant Human Kallikrein 3/PSA His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98962

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NBP1-98962-0.5mg
NBP1-98962-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Kallikrein 3/PSA Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-261 of Human Kallikrein 3/PSA

Source: E.coli

Amino Acid Sequence: IVGGW ECEKHSQPWQ VLVASRGRAV CGGVLVHPQW VLTAAHCIRN KSVILLGRHS LFHPEDTGQV FQVSHSFPHP LYDMSLLKNR FLRPGDDSSH DLMLLRLSEP AELTDAVKVM DLPTQEPALG TTCYASGWGS IEPEEFLTPK KLQCVDLHVI SNDVCAQVHP QKVTKFMLCA GRWTGGKSTC SGDSGGPLVC NGVLQGITSW GSEPCALPER PSLYTKVVHY RKWIKDTIVA NP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Kallikrein 3/PSA His Protein

SDS-PAGE: Recombinant Human Kallikrein 3/PSA His Protein [NBP1-98962]

SDS-PAGE: Recombinant Human Kallikrein 3/PSA His Protein [NBP1-98962]

SDS-Page: Recombinant Human Kallikrein 3/PSA His Protein [NBP1-98962] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-98962
Formulation 20 mM Tris-HCl buffer (pH8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kallikrein 3/PSA

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This protein is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Recombinant human KLK3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

KLK3, PSA

Gene Symbol

KLK3

Additional Kallikrein 3/PSA Products

Product Documents for Recombinant Human Kallikrein 3/PSA His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Kallikrein 3/PSA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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