Recombinant Mouse IL-28A/IFN-lambda 2 Protein

Name: Recombinant Mouse IL-28A/IFN-lambda 2 Protein
Brand: Novus Biologicals
SKU: NBP2-35074

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35074

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-28A/IFN-lambda 2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to IL-28A/IFN-lambda 2 Source: E. coli

Uniprot ID: Q4VK74

Amino Acid Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-28A/IFN-lambda 2 as determined by LAL method.

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL28A Protein is fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

SDS-PAGE: Recombinant Mouse IL-28A/IFN-lambda 2 Protein [NBP2-35074]

SDS-Page: Mouse IL-28A/IFN-lambda 2 Protein [NBP2-35074]

Formulation, Preparation and Storage

NBP2-35074

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-28A/IFN-lambda 2

Interferon lambda-2, as known as IL-28A, belongs to the lambda interferon family. IL-28A (IFN-lambda2), IL-28B (IFN-lambda3) and IL-29 (IFN-lambda1) are type III interferons that are class II cytokine receptor ligands. These IFNs signal through a receptor complex consisting of IL10R2 (also called CRF2-4) and IFNLR1 (also called CRF2-12). Interferon lambda-2 displays potent antiviral activity and antitumor activity, so it plays a significant role in the antiviral immune defense in the intestinal epithelium. It also upregulate MHC class I antigen expression. Recombinant murine interferon lambda-2 contains 174 amino acids and it shares 81 % and 66 % a.a. sequence identity with rat and human IFN-lambda2, respectively, and functions across species.

Long Name

Interleukin 28A

Alternate Names

IFN-lambda 2, IL28A, IFN-lambda 2/IL-28A

Gene Symbol

IFNL2

Additional IL-28A/IFN-lambda 2 Products

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Product Specific Notices for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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