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Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-34937

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NBP2-34937-100ug
NBP2-34937-1mg
NBP2-34937-500ug
NBP2-34937-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli

Uniprot ID: O18999

Amino Acid Sequence: HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha-dependent proliferation of murine D10S cells is less than 3.0 ug/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1alpha.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein

SDS-Page: Equine IL-1ra/IL-1F3/IL1RN Protein [NBP2-34937]

Formulation, Preparation and Storage

NBP2-34937
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-1ra/IL-1F3

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1beta and 19 % homology to IL-1alpha. It also shares 78 %~80 % a.a. sequence identity with murine, rat, porcine IL-1RA.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

UniProt

Additional IL-1ra/IL-1F3 Products

Product Documents for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Equine IL-1ra/IL-1F3/IL1RN Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Reconstitution Calculator

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