Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF

Catalog # 2488-PI | R&D Systems, Inc. a Bio-Techne Brand
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2488-PI-200
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Key Product Details

Source

Human Plasma

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Human plasma-derived Serpin G1/C1 Inhibitor protein
The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

NPNATSSS

SDS-PAGE

93 kDa, reducing conditions

Activity

Measured by its ability to inhibit Recombinant Human Complement Component C1s (Catalog # 2060-SE) cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl).
The IC50 is

Formulation, Preparation and Storage

2488-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile, deionized water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin G1/C1 Inhibitor

As a member of the serpin superfamily of serine protease inhibitors, Serpin G1/C1 inhibitor is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). Serpin G1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).

References

  1. Davis, A.E. III et al. (1993) Methods Enzymol. 223:97.
  2. Davis, A.E. III (2004) Drug News Perspect. 17:439.

Alternate Names

C1 Inhibitor

Entrez Gene IDs

710 (Human)

Gene Symbol

SERPING1

Product Documents for Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF

Certificate of Analysis

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Product Specific Notices for Human Serpin G1/C1 Inhibitor (Plasma) Protein, CF

For research use only

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