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Human Fibronectin Protein, CF Best Seller

Catalog # 1918-FN | R&D Systems, Inc. a Bio-Techne Brand
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1918-FN-02M

Key Product Details

Source

Human Plasma

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human plasma-derived Fibronectin protein
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells.
The ED50 for this effect is 35.0-420 ng/mL.

Scientific Data Images for Human Fibronectin Protein, CF

Human Fibronectin Protein Bioactivity.

Human Fibronectin (Catalog # 1918-FN) supports the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is 35.0-420 ng/mL.

Formulation, Preparation and Storage

1918-FN
Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after opening.
Avoid vortexing and excessive agitation.

Background: Fibronectin

Fibronectin (Fn) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matirx (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed Fn type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble Fibronectin to Fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of Fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

References

  1. Johansson, S. et al. (1997) Frontiers in Bioscience 2:126.
  2. Geiger, B. et al. (2001) Nature Reviews Molecular Cell Biology 2:793.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human Fibronectin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Fibronectin Protein, CF

For research use only

Citations for Human Fibronectin Protein, CF (34)

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Customer Reviews for Human Fibronectin Protein, CF (5)

4.6 out of 5
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  • Human Fibronectin Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 07/28/2022
    Human Fibronectin Protein, CF 1918-FN
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 06/15/2022
    Human Fibronectin Protein, CF 1918-FN
  • Human Fibronectin Protein, CF
    Name: Irving Garcia
    Application: Cell Adhesion
    Verified Customer | Posted 09/29/2020
  • Human Fibronectin Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/04/2019
    Human Fibronectin Protein, CF 1918-FN
  • Human Fibronectin Protein, CF
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 02/05/2018

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FAQs for Human Fibronectin Protein, CF

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  • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

  • Q: What is the molecular weight of Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    A: This Human Fibronectin Protein is purified from human plasma. There are multiple isoforms of fibronectin that vary in molecular weight. Due to the varied weight of the different isoforms, the MW of purified fibronectin can run between 180-285 kDa in an SDS-PAGE gel under reducing conditions.

  • Q: The storage recommendation listed on the CoA and datasheet for Human Fibronectin Protein, CF (catalog # 1918-FN) says to store in a manual defrost freezer, yet store at 2 to 8 °C. Can 1918-FN be frozen?

    A: Catalog #1918-FN should be stored exclusively at 2 to 8 °C and never frozen. We are working to correct the language about the manual defrost freezer.

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