Recombinant Human Hsp47 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22576

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HSP47 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 18-418 of Human Hsp47

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Hsp47 His Protein

SDS-PAGE: Recombinant Human Hsp47 His Protein [NBC1-22576]

SDS-PAGE: Recombinant Human Hsp47 His Protein [NBC1-22576]

SDS-Page: Recombinant Human Hsp47 Protein [NBC1-22576]

Formulation, Preparation and Storage

NBC1-22576
Formulation 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP47

Description: Heat shock protein 47(HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

Additional HSP47 Products

Product Documents for Recombinant Human Hsp47 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Hsp47 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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