Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # FL-1201-NB
Key Product Details
Conjugate
FITC (Excitation= 495 nm, Emission= 519 nm)
Applications
Bioactivity, Immunocytochemistry/ Immunofluorescence
Product Specifications
Description
A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.
Source: Griffonia (Bandeiraea) simplicifolia seeds
GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.
Fluorescein labeled GSL I-B4 has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorescein and is preserved with sodium azide. Excitation maximum = 495 nm; emission maximum = 515 nm. Color = green.
Purity
Affinity purified
Predicted Molecular Mass
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
Application Notes
For most applications, the recommended concentration range is 5 - 20 ug/mL. For immunofluorescence applications, it is recommended that sections be mounted in VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) or VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI (H-1500-NB)..
Protein / Peptide Type
Native Protein
Formulation, Preparation and Storage
FL-1201-NB
| Formulation | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mM Ca2+ |
| Preservative | 0.08% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C in the dark. |
Background: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
Additional Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Products
Product Documents for Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein
Product Specific Notices for Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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