Flagellin Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18154
Key Product Details
Product Specifications
Description
Source: Salmonella typhimurium strain 14028
Uniprot ID: P06179
Purity
>90% SDS-PAGE
Endotoxin Level
<0.1EU/ug purified protein (LAL test; Lonza).
Activity
Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivoassays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice.
TLR5 can be stimulated in vitro by Flagellin 5-50ng /ml & in vivo at a starting concentration of 0.1-1 ug /mouse.)
TLR5 can be stimulated in vitro by Flagellin 5-50ng /ml & in vivo at a starting concentration of 0.1-1 ug /mouse.)
Specificity
Binds to human and mouse TLR5 (Toll-like receptor 5).
Protein / Peptide Type
Native Protein
Scientific Data Images for Flagellin Native Protein
![ELISA: Flagellin Native Protein [NBP3-18154]](https://resources.bio-techne.com/images/products/Flagellin-Native-Protein-ELISA-NBP3-18154-img0001.jpg "ELISA: Flagellin Native Protein [NBP3-18154]")
ELISA: Flagellin Native Protein [NBP3-18154]
ELISA: Flagellin Native Protein [NBP3-18154] - Flagellin activates TLR5.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin. Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.Formulation, Preparation and Storage
NBP3-18154
| Formulation | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 100 ul sterile water (1mg/ml after reconstitution) |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Flagellin
UniProt
Additional Flagellin Products
Product Specific Notices for Flagellin Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...