Flagellin Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18149
Key Product Details
Product Specifications
Description
Source: Salmonella typhimurium strain 14028
Uniprot ID: P06179
Purity
>95% pure by SDS-PAGE
Endotoxin Level
<0.01EU/ug purified protein (LAL test; Lonza).
Activity
Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/ml & in vivo at a starting concentration of 0.1-1ug /mouse.
Specificity
Binds to human and mouse TLR5 (Toll-like receptor 5).
Protein / Peptide Type
Native Protein
Scientific Data Images for Flagellin Native Protein
![ELISA: Flagellin Native Protein [NBP3-18149]](https://resources.bio-techne.com/images/products/Flagellin-Native-Protein-ELISA-NBP3-18149-img0001.jpg "ELISA: Flagellin Native Protein [NBP3-18149]")
ELISA: Flagellin Native Protein [NBP3-18149]
ELISA: Flagellin Native Protein [NBP3-18149] - Flagellin activates TLR5 in vivo.Method: Mice WT are injected i.v. with indicated doses of Flagellin. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin, but no expression of CCL20 and IL-6 could be observed (not shown).Formulation, Preparation and Storage
NBP3-18149
| Formulation | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Flagellin
UniProt
Additional Flagellin Products
Product Specific Notices for Flagellin Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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