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Recombinant Human FGF basic/FGF2/bFGF Protein

Catalog # NBP2-34921 | Novus Biologicals a Bio-Techne Brand
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NBP2-34921-100ug
NBP2-34921-10ug
NBP2-34921-1mg
NBP2-34921-500ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 147 amino acids corresponding to FGF basic/FGF2/bFGF Source: E. coli

Uniprot ID: P09038

Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF basic/FGF2/bFGF as determined by LAL method.

Predicted Molecular Mass

16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FGF basic/FGF2/bFGF Protein

SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]

Formulation, Preparation and Storage

NBP2-34921
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 0.02% Tween-20, pH 7.6.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

Human FGF-2 (bFGF), encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human FGF-2 (bFGF) shares 54 % amino acid sequence identity with aFGF. Affinity between FGF-2 (bFGF) and its receptors can be increased by heparin or heparan sulfate proteoglycan. FGF-2 (bFGF) plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF-2 (bFGF) are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-2 (bFGF) is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

UniProt

Product Documents for Recombinant Human FGF basic/FGF2/bFGF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human FGF basic/FGF2/bFGF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human FGF basic/FGF2/bFGF Protein (7)

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Customer Reviews for Recombinant Human FGF basic/FGF2/bFGF Protein (2)

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  • Human FGF basic/FGF2 Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/04/2017
    SDS PAGE gel with Coomassie blue staining showing detection of Human FGF basic/FGF 2 Protein ( NBP2-34921) (0.5 ug) at about 17 kDa
    Recombinant Human FGF basic/FGF2/bFGF Protein NBP2-34921
  • Human FGF basic/FGF2 Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 01/04/2017
    SDS PAGE gel with Coomassie blue staining showing detection of 0.5 ug Human FGF basic protein (NBP2-34921-10 ug)
    Recombinant Human FGF basic/FGF2/bFGF Protein NBP2-34921

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