Recombinant Human eIF4H His Protein

Name: Recombinant Human eIF4H His Protein
Brand: Novus Biologicals
SKU: NBP1-98965

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98965

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all eIF4H Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-248 of Human eIF4H

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

29.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human eIF4H His Protein

SDS-PAGE: Recombinant Human eIF4H His Protein [NBP1-98965]

SDS-Page: Recombinant Human eIF4H Protein [NBP1-98965] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98965

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 50% glycerol, 2 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4H

Eukaryotic translation initiation factor 4H, also known as EIF4H, is a 248 amino acid protein that localizes to the perinuclear region of the cytoplasm and is expressed as two isoforms, designated short and long. EIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A and EIF4B. EIF4H was further shown to stimulate the initial rate and extent of EIF4A-mediated mRNA secondary structure unwinding. Defects in the gene encoding EIF4H are associated with Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities. Recombinant human EIF4H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

eukaryotic translation initiation factor 4H, KIAA0038eIF-4H, WBSCR1, Williams-Beuren syndrome chromosome region 1, WSCR1Williams-Beuren syndrome chromosomal region 1 protein

Gene Symbol

EIF4H

Additional eIF4H Products

Product Documents for Recombinant Human eIF4H His Protein

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COA

Product Specific Notices for Recombinant Human eIF4H His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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