Recombinant eGFP His (N-Term) His (C-Term) Protein

Name: Recombinant eGFP His (N-Term) His (C-Term) Protein
Brand: Novus Biologicals
SKU: NBP1-99915

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99915

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His (N-Term), His (C-Term)

Conjugate

Unconjugated

Applications

Functional Assay, SDS-PAGE

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Product Specifications

Description

A recombinant protein with a N-Terminal and a C-terminal His-tag and corresponding to eGFP

Source: E.coli Amino Acid Sequence: U57607.1 (NCBI Nucleotide)

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab51992)

Purity

>97%, by SDS-PAGE

Endotoxin Level

<0.1 ng/ug

Predicted Molecular Mass

32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Endotoxin level <0.1 ng/ug; suitable for in vivo injection or cell culture applications.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP1-99915

Preparation Method	Determined to be >97% pure by SDS-PAGE
Formulation	Freeze Dried
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with PBS to 1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: eGFP

The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody and GFP antibody on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm.

Alternate Names

Enhanced Green Fluorescent Protein, GFP, Green Fluorescent Protein

Additional eGFP Products

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COA

Product Specific Notices for Recombinant eGFP His (N-Term) His (C-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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