Recombinant Human DNA Polymerase beta His Protein

Name: Recombinant Human DNA Polymerase beta His Protein
Brand: Novus Biologicals
SKU: NBP1-72434

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72434

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Product Datasheet / COA

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all DNA Polymerase beta Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-335 of Human DNA Polymerase beta

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DNA Polymerase beta His Protein

SDS-PAGE: Recombinant Human DNA Polymerase beta His Protein [NBP1-72434]

SDS-Page: Recombinant Human DNA Polymerase beta Protein [NBP1-72434] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72434

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase beta

DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

POLB

Gene Symbol