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Recombinant Human Cyr61/CCN1 Protein

Catalog # NBP2-34944 | Novus Biologicals a Bio-Techne Brand
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NBP2-34944-100ug
NBP2-34944-1mg
NBP2-34944-500ug
NBP2-34944-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

SDS-PAGE, Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 357 amino acids corresponding to Cyr61/CCN1 Source: E. coli

Uniprot ID: O00622

Amino Acid Sequence: TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Cyr61/CCN1 as determined by LAL method.

Predicted Molecular Mass

39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 ug/ml, corresponding to a specific activity of > 330 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cyr61/CCN1 Protein

SDS-Page: Human Cyr61/CCN1 Protein [NBP2-34944]

Formulation, Preparation and Storage

NBP2-34944
Formulation Lyophilized from a 0.2 um filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Cyr61/CCN1

Cysteine-rich angiogenic inducer 61 (Cyr61) encoded by the Cyr61 gene is a dynamically expressed, multifunctional matricellular protein and it is also a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family. Cyr61 plays essential roles in cardiovascular development during embryogenesis and regulates inflammation, wound healing and fibrogenesis in the adult. Aberrant CCN1 expression is associated with myriad pathologies, including various cancers and diseases associated with chronic inflammation. Mature human Cyr61 shares 93 % amino acid sequence identity with mouse and rat Cyr61. Cyr61 consists of four domains. There are an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Gene Symbol

CCN1

UniProt

Additional Cyr61/CCN1 Products

Product Documents for Recombinant Human Cyr61/CCN1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Cyr61/CCN1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Reconstitution Calculator

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