Recombinant Human Cullin 1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49441

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NBP1-49441-0.05mg
NBP1-49441-0.25mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CUL1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-410 of Human Cullin 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

49.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cullin 1 His Protein

SDS-PAGE: Recombinant Human Cullin 1 His Protein [NBP1-49441]

SDS-PAGE: Recombinant Human Cullin 1 His Protein [NBP1-49441]

SDS-Page: Cullin 1 Protein [NBP1-49441] - 15% SDS-PAGE (3 ug protein loaded).

Formulation, Preparation and Storage

NBP1-49441
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 100 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CUL1

Cullin1, also known CUL1, is a core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Recombinant human CUL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Cullin 1

Alternate Names

Cullin 1

Gene Symbol

CUL1

Additional CUL1 Products

Product Documents for Recombinant Human Cullin 1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cullin 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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