Recombinant Mouse Bcl-xL Protein

Name: Recombinant Mouse Bcl-xL Protein
Brand: Novus Biologicals
SKU: NBP2-35141

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35141

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Product Datasheet / COA

Catalog #

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q64373

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Bcl-xL Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 211 amino acids corresponding to Bcl-xL Source: E. coli

Uniprot ID: Q64373

Amino Acid Sequence: SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW IQENGGWDTF VDLYGNNAAA ESRKGQERFN R

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Bcl-xL as determined by LAL method.

Predicted Molecular Mass

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Test in Process.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Bcl-xL Protein

SDS-PAGE: Recombinant Mouse Bcl-xL Protein [NBP2-35141]

SDS-Page: Mouse Bcl-xL Protein [NBP2-35141]

Formulation, Preparation and Storage

NBP2-35141

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Bcl-xL

Bcl-X, also named as BCL2L1 or BCL2L, belongs to the Bcl-2 family and is encoded by the BCL2L1 gene in human. Alternative splicing of Bcl-X results in at least two isoforms, isoform Bcl-X(L) (also named as Bcl-xL) and isoform Bcl-x(S) (also named as Bcl-xS). Bcl-xL is found in tissues containing long-lived postmitotic cells, such as adult brain, while Bcl-xS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Bcl-X forms homodimer or heterodimer with other Bcl-2 proteins, like BAK, BAX or Bcl-2, to act as anti- or pro- apoptotic regulators. Bcl-xL appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane, and it also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. In contrast, Bcl-xS is a pro-apoptotic protein that promotes apoptosis.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Gene Symbol

BCL2L1

UniProt