Recombinant Human APE T7 Protein

Name: Recombinant Human APE T7 Protein
Brand: Novus Biologicals
SKU: NBP1-72280

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72280

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all APE Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 1-318 of Human APE

Source: E.coli

Amino Acid Sequence: MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AAGEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGLASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPLADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSKIRSKALG SDHCPITLYL AL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human APE T7 Protein

SDS-PAGE: Recombinant Human APE T7 Protein [NBP1-72280]

SDS-Page: Recombinant Human APE Protein [NBP1-72280] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72280

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 2 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APE

APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Apurinic/Apyrimidinic Endonuclease

Alternate Names

APEX1

Gene Symbol