Recombinant Human AADACL1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23283

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NBP2-23283
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all AADACL1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-275 of Human AADACL1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEELNA VIVSIEYRLV PKVYFPEQIH DVVRATKYFL KPEVLQKYMV DPGRICISGD SAGGNLAAAL GQQFTQDASL KNKLKLQALI YPVLQALDFN TPSYQQNVNT PILPRYVMVK YWVDYFKGNY DFVQAMIVNN HTSLDVEEAA AVRARLNWTS LLPASFTKNY KPVVQTTGNA RIVQELPQLL DARSAPLIAD QAVLQLLPKT YILTCEHDVL RDDGIMYAKR LESAGVEVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY IKWLDQNL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AADACL1 His Protein

SDS-PAGE: Recombinant Human AADACL1 His Protein [NBP2-23283]

SDS-PAGE: Recombinant Human AADACL1 His Protein [NBP2-23283]

SDS-Page: AADACL1 Protein [NBP2-23283]

Formulation, Preparation and Storage

NBP2-23283
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AADACL1

Neutral cholesterol ester hydrolase 1, also known as NCEH1, hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. It may be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also NCEH1 is involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. This protein may contribute to cancer pathogenesis by promoting tumor cell migration. Recombinant human NCEH1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Neutral cholesterol ester hydrolase 1

Alternate Names

EC 3.1.1.-, EC 3.1.1.71, KIAA1363, NCEH, NCEH1

Gene Symbol

NCEH1

Additional AADACL1 Products

Product Documents for Recombinant Human AADACL1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human AADACL1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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