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TRACP/PAP/ACP5 Overexpression Lysate

Catalog # NBP2-09379 | Novus Biologicals a Bio-Techne Brand
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NBP2-09379

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for TRACP/PAP/ACP5 Overexpression Lysate

TRACP/PAP/ACP5 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC225473

Accession#: NM_001111036

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

34.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for TRACP/PAP/ACP5 Overexpression Lysate

Western Blot: ACP5 Overexpression Lysate (Adult Normal) [NBP2-09379] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ACP5.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: TRACP/PAP/ACP5

TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.

Long Name

Tartrate Resistant Acid Phosphatase 5

Alternate Names

ACP5, TRACP-5b, TTRRAP

Gene Symbol

ACP5

Additional TRACP/PAP/ACP5 Products

Product Documents for TRACP/PAP/ACP5 Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRACP/PAP/ACP5 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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