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Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Catalog # NB820-59657 | Novus Biologicals a Bio-Techne Brand
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NB820-59657

Key Product Details

Species

Mouse

Applications

Immunoprecipitation, SDS-PAGE, Western Blot

Product Summary for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Product Specifications

Applications

Western Blot (10 ug- 20 ug)

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Mouse Normal Tissue: Brain

Type

Tissue

Life Stage

Adult

Tissue

Brain

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Scientific Data Images for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].
SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].
SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Brain

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Product Documents for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

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  • Q: Can this product be used as a positive control for anti-GluR2 (C-Term) and anti-GluR3 (N-term) antibodies?

    A: We haven't tested these lysates for the specific expression of any single target, but I would expect GLUR2 and GLUR3 to be present. The only concern I have is how highly expressed these proteins would be in these samples and if they're abundant enough to serve as a good positive control. The donors for NB820-59657 vary, and you may see some change in signal if you were to probe multiple lots. I think that the best positive control (depending on your experiment) may be an over-expression lysate or recombinant protein.

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