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Human Brain Whole Tissue Lysate (Adult Whole Normal)

Catalog # NB820-59177 | Novus Biologicals a Bio-Techne Brand
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NB820-59177

Key Product Details

Species

Human

Applications

ELISA, Western Blot

Product Summary for Human Brain Whole Tissue Lysate (Adult Whole Normal)

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Product Specifications

Applications

Western Blot (10 ug - 20 ug)

Application Notes

Use in ELISA reported in scientific literature (PMID 27120609).

Specificity

Total Protein - Human Adult Normal Tissue: Brain

Type

Tissue

Life Stage

Adult

Tissue

Brain

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Scientific Data Images for Human Brain Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Brain

This lysate was produced from total protein - Human Adult Normal Tissue: Brain. Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Product Documents for Human Brain Whole Tissue Lysate (Adult Whole Normal)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Brain Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Human Brain Whole Tissue Lysate (Adult Whole Normal) (27)

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Customer Reviews for Human Brain Whole Tissue Lysate (Adult Whole Normal) (1)

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  • Name: Ming Lu
    Application: Western Blot
    Sample Tested: Human Brain Whole Tissue Lysate (Adult Normal), Novus NB820-59177
    Species: Human
    Verified Customer | Posted 10/03/2014
    Human Brain Whole Tissue Lysate (Adult Whole Normal) NB820-59177

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FAQs for Human Brain Whole Tissue Lysate (Adult Whole Normal)

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  • Q: I have a question regarding your product no. NB820-59177. I would like to use this lysate in an ELISPOT assay and I am not sure if this is possible. My particular question is if this product is sterile, i.e. can it be used for cell culture?

    A: Unfortunately we have not tested this lysate for use in an ELISPOT assay or for use in cell culture. I would estimate this product not to be suitable for your assay as the buffer will contain a host of protease inhibitors and detergent. Buffer information: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 with MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors. As such live cells probably would not do well in contact with this buffer.

  • Q: May we ask how much we shall load by using NB820-59177 as positive control in WB?

    A: We recommend loading 10-20ug of lysate per well for Western blot.

  • Q: Is there a recommended expiration date for the Human Brain Whole Tissue Lysate, cat:NB820-59177, Lot: B703069?

    A: You should store at 2-8 degrees Celsius for continuous use within a day. For extended storage, freeze working aliquots at -70 degrees Celsius. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is six months from the date of receipt.

  • Q: May we ask if the Human Brain Whole Tissue Lysate (cat. no. NB820-59177) is suitable for RNA extraction?

    A: Unfortunately, we have not yet tested NB820-59177 in any sort of RNA extraction, so I do not have any data supporting that application. We have only used this as a control in WB and IP.

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