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A-549 Nuclear Cell Lysate, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # L025V3

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L025V3

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for A-549 Nuclear Cell Lysate

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Nuclear extract cell lysate (denatured)

Product Specifications

Application Notes

The lysate is ready to load on SDS-PAGE for Western blotting.

Specificity

A-549 (human lung carcinoma) nuclear extract lysate (denatured)

Localization

Lung

Type

Cell

Tissue

Lung

Protein State

Denatured

Subcellular Fraction

Nuclear

Scientific Data Images for A-549 Nuclear Cell Lysate

Formulation, Preparation, and Storage

Preparation Method

Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95C for 5 min, and cooled rapidly.

Formulation

In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).

Concentration

2 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: A-549

Additional A-549 Products

Product Documents for A-549 Nuclear Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for A-549 Nuclear Cell Lysate

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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