Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Name: Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control
Brand: R&D Systems
SKU: IC003R

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC003R

Citations (3)

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Immunogen:	KLH
Applications:	Control
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Source:	Monoclonal Mouse IgG_2A Clone # 20102
Specificity:	Selected for use as mouse IgG_2A isotype control for flow cytometry applications.

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Applications for Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

	Recommended Concentration	Sample
Control	Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G2A Fc
Alternate Names	IgG2A

Citations for Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

L El-Shennaw et al. (2022), Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 13(1):405.
PMID: 35058437
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

T Lowin et al. (2022), Impact of delta9-Tetrahydrocannabinol on Rheumatoid Arthritis Synovial Fibroblasts Alone and in Co-Culture with Peripheral Blood Mononuclear Cells Biomedicines, 10(5).
PMID: 35625855
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

RB Hansen et al. (2021), Leukocyte TNFR1 and TNFR2 Expression Contributes to the Peripheral Immune Response in Cases with Ischemic Stroke Cells, 10(4).
PMID: 33918875

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Product Documents for Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

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Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

R&D Systems, Inc. a Bio-Techne Brand

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