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Mouse IgG2a Isotype Control (M2A)

Catalog # NBP1-96778 | Novus Biologicals a Bio-Techne Brand

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M2A

Format

Purified

Concentration

Please contact technical services for concentration.

Product Summary for Mouse IgG2a Isotype Control (M2A)

Specificity

Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Mouse IgG2a Isotype Control (M2A)

Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 cells treated for 3h with vehicle or perifosine (0.5, 1 or 2.5 uM) were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.
Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 BC cells either untreated or treated with sorafenib (20 uM) for 1 or 3 h were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (anti-pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.
Mouse IgG2a Isotype Control (M2A)

Mouse IgG2a Isotype Control (M2A)

SDS-Page Results of Mouse IgG2a Isotype Control (M2A).Lane 1: Mouse IgG2a Isotype Control (M2A) Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker

Applications for Mouse IgG2a Isotype Control (M2A)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:1000 - 1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using NBP1-96778 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2a Isotype Control (M2A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IgG2a Isotype Control (M2A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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FAQs for Mouse IgG2a Isotype Control (M2A)

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  • Q: I would like to know the clonality of this sample. Is this monoclonal or polyclonal?

    A: Their is no clonality for this product as it is an isotype control, and contains multiple different non-specific IgG2a immunoglobulins that are non-specific, to rule out binding with Fc receptors in flow and other experiments requiring this type of control.

  • Q: I want to know how the Ab was purified. More specifically, if it was with chomatography (like anti-mouse IgG columns, or other) OR with protein A or protein G columns. The latter is important to us to take presence of trace amounts of protein A or protein G into consideration.

    A: This sample is a mouse IgG2a Isotype Control, which has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.

Showing  1 - 2 of 2 FAQs Showing All
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