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Better as one

Key Product Details

Species Reactivity

Rat

Applications

Control, ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # KLH/G2a-1-1

Format

Low Endotoxin and Azide Free

Concentration

Please contact technical services for concentration.

Product Summary for Rat IgG2a Isotype Control (KLH/G2a-1-1)

Immunogen

KLH

Specificity

KLH

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Description

This Rat IgG2A (KLH/G2a-1-1) immunoglobulin is useful as an isotype control for Rat IgG2A antibody.

Scientific Data Images for Rat IgG2a Isotype Control (KLH/G2a-1-1)

Flow Cytometry: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Immunofluorescence: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.

Applications for Rat IgG2a Isotype Control (KLH/G2a-1-1)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Paraffin

1:10 - 1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

PBS.

Format

Low Endotoxin and Azide Free

Preservative

No Preservative

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Rat IgG2a Isotype Control (KLH/G2a-1-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IgG2a Isotype Control (KLH/G2a-1-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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