YADA
Tocris Bioscience, part of Bio-Techne | Catalog # 6650
Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Key Product Details
Description
Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Product Description
YADA is a green-yellow lucifer yellow-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Exhibits large stokes shift and wider emission spectra than other FDAAs, allowing excitation via a violet light source and detection with a green filter. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~426/535 nm.Optical Data
| Emission Color | Green-yellow |
| λabs | 426 nm |
| λem | 535 nm |
| Extinction Coefficient (ε) | 24200 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Application | Confocal Microscopy, Super Resolution Microscopy |
Spectra Viewer
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Spectra ViewerProduct Specifications
Molecular Weight
459.41
Formula
C15H13N3O10S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(αR)-α,6-Diamino-1,3-dioxo-5,8-disulfo-1H-benz[de]isoquinoline-2(3H)-propanoic acid
CAS Number
1471982-33-8
PubChem ID
134159307
InChI Key
YXWYHJBYHLJEIR-UHFFFAOYSA-N
SMILES
O=C(N(C[C@@H](N)C(O)=O)C1=O)C2=C(C1=CC(S(=O)(O)=O)=C3N)C3=CC(S(=O)(O)=O)=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.94 | 100 |
Preparing Stock Solutions
for YADA
The following data is based on the product molecular weight 459.41
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.18 mL | 10.88 mL | 21.77 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.35 mL |
| 10 mM | 0.22 mL | 1.09 mL | 2.18 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665
Product Specific Notices for YADA
For research use only
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