TBI
Tocris Bioscience, part of Bio-Techne | Catalog # 7660
Fluorogenic ligand for Broccoli RNA aptamer
Key Product Details
Description
Fluorogenic ligand for Broccoli RNA aptamer
Product Description
TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (KD values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M-1 cm-1.Licensing Information
Sold under license from LucernaOptical Data
| Emission Color | Green |
| λabs | 485 nm |
| λem | 527 nm |
| Extinction Coefficient (ε) | 35100 M-1cm-1 |
| Quantum Yield (φ) | 0.64 |
| Application | Live-cell Imaging, Fluorescence Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
386.38
Formula
C18H12F2N4O2S
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one
CAS Number
2863639-29-4
InChI Key
FFVXYOZVQLWDIF-NSIKDUERSA-N
SMILES
FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.73 | 20 |
Preparing Stock Solutions
for TBI
The following data is based on the product molecular weight 386.38
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 12.94 mL | 64.70 mL | 129.41 mL |
| 1 mM | 2.59 mL | 12.94 mL | 25.88 mL |
| 2 mM | 1.29 mL | 6.47 mL | 12.94 mL |
| 10 mM | 0.26 mL | 1.29 mL | 2.59 mL |
Calculators
Molarity Calculator
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Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Li Engineering fluorophore recycling in a fluorogenic RNA aptamer. Angew.Chem.Int.Ed.Engl. 2021 PMID: 34490956
Product Specific Notices for TBI
For research use only
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