RMR-Tre
Tocris Bioscience, part of Bio-Techne | Catalog # 8013
Far-red fluorogenic trehalose probe for live mycobacteria imaging
Key Product Details
Description
Far-red fluorogenic trehalose probe for live mycobacteria imaging
Product Description
RMR-Tre is a far-red fluorogenic trehalose specific probe for live mycobacteria imaging. It enables fast, no-wash, low-background fluorescence detection of live mycobacterial outer membrane. RMR-Tre contains a molecular rotor fluorophore that brightly emits signal in the far-red region upon trehalose-guided metabolic integration into the mycomembrane. It exhibited up to a 100- fold enhancement in Mycobacterium tuberculosis labeling compared to existing fluorogenic trehalose probes. Excitation and emission maxima (λ) are 549 nm and 671 nm, respectively.Scientific Data Images for RMR-Tre
Application of RMR-Tre.
Image shows fluorescence detection of live mycobacterial outer membrane: M. smegmatis were incubated for 1 h in the presence of 100 μM of RMR-Tre, then directly imaged by fluorescence microscopy without washing. Scale bar: 10 μm.Licensing Information
Sold under license from Central Michigan UniversityOptical Data
| Emission Color | Far Red |
| λabs | 536 nm |
| λem | 684 nm |
| Closest Laser line | 540 nm |
Spectra Viewer
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Product Specifications
Molecular Weight
686.73
Formula
C32H38N4O11S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3-(6-(5-(2,2-Dicyanovinyl)thiophen-2-yl)-3,4-dihydroquinolin-1(2H)-yl)-N-(((2R,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl)propanamide
PubChem ID
172005912
InChI Key
SSAVIDOUSMJZDG-IVJDZZAPSA-N
SMILES
O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]1CO)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CNC(CCN3C4=C(CCC3)C=C(C5=CC=C(S5)/C=C(C#N)/C#N)C=C4)=O)O)O)O)O)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.87 | 10 |
Preparing Stock Solutions
for RMR-Tre
The following data is based on the product molecular weight 686.73
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.56 mL | 72.81 mL | 145.62 mL |
| 0.5 mM | 2.91 mL | 14.56 mL | 29.12 mL |
| 1 mM | 1.46 mL | 7.28 mL | 14.56 mL |
| 5 mM | 0.29 mL | 1.46 mL | 2.91 mL |
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Background References
References are publications that support the biological activity of the product.
- Banahene A far-red molecular rotor fluorogenic trehalose probe for live mycobacteria detection and drug-susceptibility testing. Angew.Chem.Int.Ed.Engl. 2023 PMID: 36346622
Product Specific Notices for RMR-Tre
For research use only
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