Propidium iodide
Tocris Bioscience, part of Bio-Techne | Catalog # 5135
Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, nuclear counterstaining, viability staining. Application: flow cytometry, confocal microscopy, fluorescence microscopy
Key Product Details
Description
Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, nuclear counterstaining, viability staining. Application: flow cytometry, confocal microscopy, fluorescence microscopy
Alternative Names
PI
Product Description
Key information: Propidium iodide (PI) is a widely used red-fluorescent DNA stain. It is membrane impermeant to live cells and so PI staining differentiates live and dead cells. PI can be used to stain nucleic acids in dead cells across different cell types, including mammalian cells, bacteria, and yeast.Application: flow cytometry, confocal microscopy, fluorescence microscopy.
Used for: apoptosis detection by PI staining, nuclear counterstaining in multicolor fluorescent assays, cell death measuring in a mixed live-cell population by PI staining and flow cytometry.
Properties and Photophysical Data: Propidium iodide fluorescence increases by approximately 20- to 30-fold upon binding to DNA or RNA (through intercalation). When unbound, excitation and emission maxima (λ) are 493 nm and 636 nm, respectively, when bound to DNA, excitation and emission maxima (λ) are 535 nm and 617 nm, respectively.
Optical Data
| Emission Color | Orange |
| λabs | 535 nm |
| λem | 617 nm |
| Application | Flow Cytometry, Fluorescence Microscopy, Confocal Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
668.39
Formula
C27H34I2N4
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
CAS Number
25535-16-4
PubChem ID
104981
InChI Key
XJMOSONTPMZWPB-UHFFFAOYSA-M
SMILES
NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.34 | 5 with sonication |
Preparing Stock Solutions
for Propidium iodide
The following data is based on the product molecular weight 668.39
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 29.92 mL | 149.61 mL | 299.23 mL |
| 0.25 mM | 5.98 mL | 29.92 mL | 59.85 mL |
| 0.5 mM | 2.99 mL | 14.96 mL | 29.92 mL |
| 2.5 mM | 0.60 mL | 2.99 mL | 5.98 mL |
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Background References
References are publications that support the biological activity of the product.
- Sonnemann p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 2014 PMID: 24281001
- Yun Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 2013 PMID: 24297180
- Jeong SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 2013 PMID: 24368766
Product Specific Notices for Propidium iodide
For research use only
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