Propidium iodide

Name: Propidium iodide
Brand: Tocris Bioscience
SKU: 5135
Rating: 5 (2 reviews)

Catalog # 5135 | Tocris Bioscience a Bio-Techne Brand

(2)

COA / SDS

Red-fluorescent DNA stain; membrane impermeant to live cells. Pi staining differentiates live and dead cells

Key Product Details

Description:	Red-fluorescent DNA stain; membrane impermeant to live cells. Pi staining differentiates live and dead cells
Alternative Names:	PI
Chemical Name:	3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Purity:	≥95% (HPLC)
Molecular Weight:	668.39

View Full Technical Details for Propidium iodide

Biological Activity for Propidium iodide

Propidium iodide is a red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.

Optical Data for Propidium iodide

Emission Color	Orange
λ_abs	535 nm
λ_em	617 nm
Application	Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Propidium iodide

M. Wt	668.39
Formula	C₂₇H₃₄I₂N₄
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	25535-16-4
PubChem ID	104981
InChi Key	XJMOSONTPMZWPB-UHFFFAOYSA-M
Smiles	NC4=CC1=C(C=C4)C3=C(C=C(N)C=C3)[N+](CCC[N+](CC)(CC)C)=C1C2=CC=CC=C2.[I-].[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Propidium iodide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	3.34	5 with sonication

Preparing Stock Solutions for Propidium iodide

The following data is based on the product molecular weight 668.39.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	29.92 mL	149.61 mL	299.23 mL
0.25 mM	5.98 mL	29.92 mL	59.85 mL
0.5 mM	2.99 mL	14.96 mL	29.92 mL
2.5 mM	0.60 mL	2.99 mL	5.98 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Propidium iodide

References are publications that support the biological activity of the product.

Sonnemann p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors. Br.J.Cancer 2014 PMID: 24281001
Jeong SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma. J.Biol.Chem. 2013 PMID: 24368766
Yun Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J.Biol.Chem. 2013 PMID: 24297180

Citations for Propidium iodide

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Propidium iodide (2)

4.5 out of 5

2 customer ratings

5 stars

50%

4 stars

50%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Good Product

Name: Rohit Sharma

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: Jurkat Cells

PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.
Propidium dye for intracellular fluorescent dye.

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.

5mM is great for visualization with an mcherry filter. It goes into water well.

There are no reviews that match your criteria.