Key Product Details
Description: | Red-fluorescent DNA stain; membrane impermeant to live cells. Pi staining differentiates live and dead cells |
Alternative Names: | PI |
Chemical Name: | 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide |
Purity: | ≥95% (HPLC) |
Molecular Weight: | 668.39 |
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Customer Reviews for Propidium iodide (2)
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Species: HumanAssay Type: In VitroCell Line/Tissue: Jurkat CellsPI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.
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Species: MouseAssay Type: Ex VivoPropidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.5mM is great for visualization with an mcherry filter. It goes into water well.
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