PA Janelia Fluor® 549, Haloalkane
Tocris Bioscience, part of Bio-Techne | Catalog # 8814
Cell-permeable photoactivatable Janelia Fluor® 549 with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag (SLT) substrate
Key Product Details
Description
Cell-permeable photoactivatable Janelia Fluor® 549 with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag (SLT) substrate
Product Description
Key Information: PA Janelia Fluor® 549, Haloalkane is a yellow cell-permeable photoactivable fluorescent dye with a chloroalkane handle for labeling fusion tag proteins.Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, super-resolution microscopy (SRM) techniques including PALM (in both live and fixed cells). Allows multicolor single tracking experiments.
Properties and Photophysical Data: PA Janelia Fluor® 549, Haloalkane shows superior brightness and photostability.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| λabs | 549 nm |
| λem | 571 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy, PALM |
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Product Specifications
Molecular Weight
684.23
Formula
C38H42ClN5O5
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
3',6'-Bis(1-azetidinyl)-N-[2-[2-[(6-chlorohexyl)oxy]ethoxy]ethyl]-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxamide
CAS Number
1811539-43-1
PubChem ID
126569721
InChI Key
MTAQLTJARYVZKC-UHFFFAOYSA-N
SMILES
ClCCCCCCOCCOCCNC(C1=CC=C(C(C23C4=CC=C(C=C4OC5=CC(N6CCC6)=CC=C35)N7CCC7)=C1)C(C2=[N+]=[N-])=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.84 | 10 |
Preparing Stock Solutions
for PA Janelia Fluor® 549, Haloalkane
The following data is based on the product molecular weight 684.23
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.61 mL | 73.07 mL | 146.15 mL |
| 0.5 mM | 2.92 mL | 14.61 mL | 29.23 mL |
| 1 mM | 1.46 mL | 7.31 mL | 14.61 mL |
| 5 mM | 0.29 mL | 1.46 mL | 2.92 mL |
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Background References
References are publications that support the biological activity of the product.
- Grimm Bright photoactivatable fluorophores for single-molecule imaging Nat.Methods 2016 PMID: 27776112
Product Specific Notices for PA Janelia Fluor® 549, Haloalkane
For research use only
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