OG 488, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 6257
Green fluorescent dye; supplied as NHS ester
Discontinued Product
6257 has been discontinued.
View all Standard Fluorescein, Coumarin and Rhodamine-based products.
Key Product Details
Description
Green fluorescent dye; supplied as NHS ester
Product Description
OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 496 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 76,000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
| Correction Factor 280 | 0.12 |
| Application | Imaging, acidic organelle probe, flow, STED |
| Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
Spectra Viewer
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Product Specifications
Molecular Weight
509.37
Formula
C25H13F2NO9
Storage
Store at -20°C
Chemical Name
2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
CAS Number
198139-51-4
PubChem ID
58720805
InChI Key
RPOLNGNGJOEMSR-UHFFFAOYSA-N
SMILES
OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 0.51 | 1 |
Preparing Stock Solutions
for OG 488, SE
The following data is based on the product molecular weight 509.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.01 mM | 196.32 mL | 981.60 mL | 1,963.21 mL |
| 0.05 mM | 39.26 mL | 196.32 mL | 392.64 mL |
| 0.1 mM | 19.63 mL | 98.16 mL | 196.32 mL |
| 0.5 mM | 3.93 mL | 19.63 mL | 39.26 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Vergene Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 1998 PMID: 9448851
Product Specific Notices for OG 488, SE
For research use only
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