Laurdan
Tocris Bioscience, part of Bio-Techne | Catalog # 7275
Polarity sensitive lipid membrane fluorescent probe. Used for: imaging lipid rafts. Application: fluorescent microscopy
Key Product Details
Description
Polarity sensitive lipid membrane fluorescent probe. Used for: imaging lipid rafts. Application: fluorescent microscopy
Product Description
Key information: Laurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). Suitable for live and fixed cells as well as in whole tissues imaging with multiphoton microscopy.Used for: imaging lipid raft and cell membrane investigations.
Application: fluorescence microscope. Suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS).
Properties and Photophysical Data: When Laurdan is incorporated into membranes, its fluorescence spectra is sensitive to the physical state of the surrounding phospholipids. Excitation and emission maxima (λ) are 366 nm and 497 nm, respectively; quantum yield = 0.61; extinction coefficient = 19,500 M-1cm-1. In gel and liquid phase membranes, excitation and emission maxima (λ) are 440 nm and 490 nm, respectively.
Optical Data
| Emission Color | Blue |
| λabs | 366 nm |
| λem | 497 nm |
| Extinction Coefficient (ε) | 19500 M-1cm-1 |
| Quantum Yield (φ) | 0.61 |
| Application | Polarity Sensitive Fluorescent Probe for Lipid Raft and Cell Membrane Investigations |
| Alternative For | C-Laurdan, Di-4-ANEPPDHQ |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
353.55
Formula
C24H35NO
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-[6-(Dimethylamino)-2-naphthalenyl]-1-dodecanone
CAS Number
74515-25-6
PubChem ID
104983
InChI Key
JHDGGIDITFLRJY-UHFFFAOYSA-N
SMILES
CCCCCCCCCCCC(C1=CC2=CC=C(N(C)C)C=C2C=C1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 7.07 | 20 |
Preparing Stock Solutions
for Laurdan
The following data is based on the product molecular weight 353.55
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 14.14 mL | 70.71 mL | 141.42 mL |
| 1 mM | 2.83 mL | 14.14 mL | 28.28 mL |
| 2 mM | 1.41 mL | 7.07 mL | 14.14 mL |
| 10 mM | 0.28 mL | 1.41 mL | 2.83 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Owen Quantitative imaging of membrane lipid order in cells and organisms. Nat.Protoc. 2011 PMID: 22157973
- Sanchez Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc.Natl.Acad.Sci.U.S.A. 2012 PMID: 22529342
Product Specific Notices for Laurdan
For research use only
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