L 012 sodium salt

Name: L 012 sodium salt
Brand: Tocris Bioscience
SKU: 5085

Catalog # 5085 | Tocris Bioscience a Bio-Techne Brand

Citations (2)

COA / SDS

Chemiluminescent ROS and RNS indicator

Key Product Details

Description:	Chemiluminescent ROS and RNS indicator
Chemical Name:	8-Amino-5-chloro-2,3-dihydro-7-phenyl-pyrido[3,4-d]pyridazine sodium salt
Purity:	≥98% (HPLC)
Molecular Weight:	310.67

View Full Technical Details for L 012 sodium salt

Technical Data
Citations (2) / Reviews

Biological Activity for L 012 sodium salt

L 012 sodium salt is a luminal-based chemiluminescent probe. Detects NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS). Active in vitro and in vivo.

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Technical Data for L 012 sodium salt

M. Wt	310.67
Formula	C₁₃H₈CIN₄NaO₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	143556-24-5
PubChem ID	78243715
InChi Key	IGEUYSJHQQCEFP-UHFFFAOYSA-M
Smiles	NC1=C3C(C(O[Na])=NNC3=O)=C(Cl)N=C1C2=CC=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for L 012 sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	6.21	20
	DMSO	31.07	100

Preparing Stock Solutions for L 012 sodium salt

The following data is based on the product molecular weight 310.67.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.22 mL	16.09 mL	32.19 mL
5 mM	0.64 mL	3.22 mL	6.44 mL
10 mM	0.32 mL	1.61 mL	3.22 mL
50 mM	0.06 mL	0.32 mL	0.64 mL

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References for L 012 sodium salt

References are publications that support the biological activity of the product.

Ichibangase Evaluation of lophine derivatives as L-012 (luminol analog)-dependent chemiluminescence enhancers for measuring horseradish peroxidase and H2 O2. Luminescence. 2013 PMID: 23630098
Zhou Noninvasive assessment of localized inflammatory responses. Free Radic.Biol.Med. 2012 PMID: 22080048
Kielland In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radic.Biol.Med. 2009 PMID: 19539751

Citations for L 012 sodium salt (2)

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Citations are publications that use Bio-Techne products. Selected citations for L 012 sodium salt include:

Mentrup et al. (2022) Phagosomal signalling of the C-type lectin receptor Dectin-1 is terminated by intramembrane proteolysis. Nat.Commun.. 13:1880
PMID: 35388002
Jie et al. (2020) IL-33/ST2 induces neutrophil-dependent reactive oxygen species production and mediates gout pain. Theranostics. 10:12189-12203
PMID: 33204337

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