Kyoto Probe-1
Tocris Bioscience, part of Bio-Techne | Catalog # 7419
Key Product Details
| Description: | Fluorescent probe that selectively identifies undifferentiated iPS/ES cells |
| Alternative Names: | KP1 |
| Chemical Name: | 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 418.35 |
Biological Activity for Kyoto Probe-1
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.
Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
Licensing Information
Sold under license from New York University
Optical Data for Kyoto Probe-1
| Emission Color | Green |
|---|---|
| λabs | 515 nm |
| λem | 529 nm |
| Quantum Yield (φ) | 0.45 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Live-cell Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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