K 114

Name: K 114
Brand: Tocris Bioscience
SKU: 3144

Tocris Bioscience a Bio-Techne Brand

Catalog # 3144

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Key Product Details

Description:	Amyloid fibril-specific fluorescent dye
Chemical Name:	4,4'-[(2-Bromo-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
Purity:	≥98% (HPLC)
Molecular Weight:	393.27

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Biological Activity for K 114

K 114 is a potent amyloid fibril-specific fluorescent dye (EC₅₀ = 20 - 30 nM). Exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ, α-synuclein and tau in situ. (Optimum wavelength = 550 nm).

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Technical Data for K 114

M. Wt	393.27
Formula	C₂₂H₁₇BrO₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	872201-12-2
PubChem ID	44828481
InChi Key	OXPHQQMZTXMEGO-RJTULKDBSA-N
Smiles	OC(C=C3)=CC=C3/C=C/C1=CC(Br)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for K 114

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	39.33	100
	ethanol	19.66	50

Preparing Stock Solutions for K 114

The following data is based on the product molecular weight 393.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.54 mL	12.71 mL	25.43 mL
5 mM	0.51 mL	2.54 mL	5.09 mL
10 mM	0.25 mL	1.27 mL	2.54 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

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References for K 114

References are publications that support the biological activity of the product.

LeVine Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114). Biochemistry 2005 PMID: 16313197
Crystal A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114. J.Neurochem. 2003 PMID: 12950445

Citations for K 114

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