Janelia Fluor® 669, NHS ester

Tocris Bioscience, part of Bio-Techne | Catalog # 6420

Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
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Key Product Details

Description

Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

Alternative Names

JF 669,NHS

View all Amine-Reactive Janelia Fluor Dyes Fluorescent Probes and Dyes »

Product Description

Key Information: Janelia Fluor® 669, NHS ester is a red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 669 nm and 682 nm, respectively; quantum yield = 0.37; extinction coefficient = 116,000 M-1cm-1; A280 correction factor = 0.043. Retains 97% fluorescence after 30 bleaching cycles.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer custom conjugation antibody services.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor® 669, SE.

Scientific Data Images for Janelia Fluor® 669, NHS ester

Applications on Tubulin for Janelia Fluor® 669, NHS ester

Applications on Tubulin for Janelia Fluor® 669, NHS ester.

Application of Janelia Fluor® 669, NHS ester dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, NHS ester. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Emission Color Far Red
Brightness
λabs 669 nm
λem 682 nm
Extinction Coefficient (ε) 116,000 M-1cm-1
Quantum Yield (φ) 0.37
Closest Laser line 640 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.043
Application Confocal Microscopy, Super Resolution Microscopy (SRM)
Alternative For Alexa Fluor® 660, Atto 655, Atto 665, CF660R

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Product Specifications

Molecular Weight

693.77

Formula

C34H30F3N3O6SSi

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

CAS Number

2127150-20-1

PubChem ID

132470916

InChI Key

QFHKALIQFJQJKE-UHFFFAOYSA-N

SMILES

C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 13.88 20

Preparing Stock Solutions for Janelia Fluor® 669, NHS ester

The following data is based on the product molecular weight 693.77

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 7.21 mL 36.03 mL 72.07 mL
1 mM 1.44 mL 7.21 mL 14.41 mL
2 mM 0.72 mL 3.60 mL 7.21 mL
10 mM 0.14 mL 0.72 mL 1.44 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 669, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 669, NHS ester

For research use only

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