Janelia Fluor® 646 Tre New
Tocris Bioscience, part of Bio-Techne | Catalog # 8967
Red fluorescent trehalose probe for live mycobacteria imaging
Key Product Details
Description
Red fluorescent trehalose probe for live mycobacteria imaging
Product Description
Key information: Janelia Fluor® 646 Tre is a red fluorescent probe for imaging Mycobacterium tuberculosis, after metabolic incorporation of the trehalose component in the lipid-rich cell envelope.Used for: imaging Mycobacterium tuberculosis in TB drug susceptibility testing.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Scientific Data Images for Janelia Fluor® 646 Tre
Application of Janelia Fluor® 646 Tre for live bacterial imaging.
Msmeg cells were incubated 4-hour at 37 ºC in the presence of 10 µM Janelia Fluor® 646 Tre. Cells were washed in DPBS and imaged using a Nikon A1R Laser Scanning Confocal Microscopy. Data kindly supplied by the Swarts lab, Central Michigan University.
Application of Janelia Fluor® 646 tre for flow cytometry using APC channel.
Msmeg cells were incubated for 4 hours and Mtb cells for 16 hours at 37 ºC in the presence of 10 µM Janelia Fluor® 646 Tre. Flow cytometry was performed on a BD Biosciences C6 Accuri™ Plus flow cytometer instrument equipped with a 640-nm red excitation and APC (675/25 nm) emission filter. Data kindly supplied by the Kamariza lab, UCLA.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Correction Factor 280 | 0.19 |
| Application | Bacterial imaging |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
819.94
Formula
C41H49N3O13Si
Storage
Store at -20°C
Purity
≥95%
Chemical Name
2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((((2R,3S,4S,5R,6R)-3,4,5-trihydroxy-6-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)tetrahydro-2H-pyran-2-yl)methyl)carbamoyl)benzoate
InChI Key
BZQLCLILAFFJOH-KEVSEYCPSA-N
SMILES
O[C@H]1[C@@H]([C@H](O[C@@H]([C@@H]1O)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CNC(C3=CC=C(C([O-])=O)C(C(C(C=CC(N4CCC4)=C5)=C5[Si]6(C)C)=C7C6=C/C(C=C7)=[N+]8CCC\8)=C3)=O)O)O)O)CO)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.2 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 646 Tre
The following data is based on the product molecular weight 819.94
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 12.20 mL | 60.98 mL | 121.96 mL |
| 0.5 mM | 2.44 mL | 12.20 mL | 24.39 mL |
| 1 mM | 1.22 mL | 6.10 mL | 12.20 mL |
| 5 mM | 0.24 mL | 1.22 mL | 2.44 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for Janelia Fluor® 646 Tre
For research use only
Loading...
Loading...